3-D Life Dextran-CD-HA Hydrogel

Catalog Number: G96-1

Unit Size: 1 Kit

Shipping Temperature: Ambient

350,00 €

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  • Description
  • Specifications

Details
Supporting Information

Description The 3-D Life Dextran-CD-HA Hydrogel Kit provides reagents for setting up hydrogels containing hyaluronic acid. Its major components are SG-Dextran and the thiol-functionalized hyaluronic acid crosslinker CD-HyLink. When SG-Dextran and CD-HyLink are combined, thiol groups on CD-HyLink form stable thioether bonds with thiol-reactive groups on SG-Dextran, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation. CD-HyLink contains a matrix metalloproteinase (MMP)-cleavable peptide sequence (Pro-Leu-Gly-Leu-Trp-Ala), which is cleaved by a broad range of MMPs including MMP1, MMP2, MMP3, MMP7, MMP9 and MMP13 [1, 2, 3]. It allows cells to locally degrade the polymer network, if they produce the indicated MMPs.

The hydrogel allows cell spreading and migration if cell adhesion peptides are present in the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups on SG-Dextran to provide a cell-adhesive matrix.

Note: Dextran hydrogels crosslinked with CD-HyLink cannot be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1).

For more information and instructions, please consult the General Protocol GP-4B "Preparation of 3-D Life Hyaluronic Acid (HA) Hydrogels containing a matrix metalloproteinase cleavage site" and the 3-D Life Hydrogels User Guide.
Catalog Number G96-1
Application 3-D cell culture, hydrogel injections, filling of microchannels, generation of soft to very stiff gels.
Quantity 1 Kit
Components
Material Quantity Concentration
SG-Dextran170 µl30 mmol/L thioreactive groups
CD-HyLink, freeze-dried2 x 200 µL10 mmol/L* thiol groups
10x CB (pH 7.2)200 µln.a.
Water2 x 1500 µln.a
*Volume/concentration after reconstitution of lyophilisate.
Reference[1] Knight, C. G. et al. FEBS 296:263-66 (1992) [2] Patterson, J. and Hubbell, J. A. Biomaterials 31, 7836-7845 (2010) [3] Deng, S. et al. J. Biol. Chem. 275, No. 40, 31422–31427 (2000)

General Protocols

Protocol 4B: Hyaluronic Acid Hydrogels CD-HA

User Guide

3-D Life Hydrogel User Guide

Data Sheet

Detailed Product Information

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