3-D Life CD-HyLink

Catalog Number: L80-3

Unit Size: 3x 200 µL

Shipping Temperature: Ambient

330,00 €

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  • Description
  • Specifications

Details
Supporting Information

Description The CD-HyLink molecule consists of hyaluronic acid carrying thiol groups along its backbone. When combined with SG-Dextran of the 3-D Life Hydrogel system, thiol groups on CD-HyLink form stable thioether bonds with the thiol-reactive groups on the polymer, which results in the formation of the hydrogel.
CD-HyLink contains a matrix metalloproteinase (MMP)-cleavable peptide sequence (Pro-Leu-Gly-Leu-Trp-Ala), which is cleaved by a broad range of MMPs including MMP1, MMP2, MMP3, MMP7, MMP9 and MMP13 [1, 2, 3]. It allows cells to locally degrade the polymer network.

Note: Dextran hydrogels crosslinked with CD-HyLink cannot be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1).

For using this product please consult General Protocol GP-4B "Preparation of 3-D Life Hyaluronic Acid (CD-HA) Hydrogels containing a matrix metalloproteinase cleavage site" and the 3-D Life Hydrogels User Guide.
Catalog Number L80-3
Application 3-D Life CD-HyLink is used together with thiol-reactive SG-Dextran of the 3-D Life Hydrogel system to establish 3-D cell cultures.
Quantity Sufficient for 4.8 ml hydrogel of a crosslinking strength of 1.2 mmol/l when used with SG-Dextran of the 3-D Life Hydrogel system.
Components
Material Quantity Concentration
CD-HyLink, freeze-dried3 x 200 µL10 mmol/L* thiol groups
Water600 µln/a
*Volume/concentration after reconstitution of lyophilisate.
Reference[1] Knight, C. G. et al. FEBS 296:263-66 (1992)
[2] Patterson, J. and Hubbell, J. A. Biomaterials 31, 7836-7845 (2010)
[3] Deng, S. et al. J. Biol. Chem. 275, No. 40, 31422–31427 (2000)

General Protocols

Protocol 4B: Hyaluronic Acid Hydrogels CD-HA

User Guide

3-D Life Hydrogel User Guide

Data Sheet

Detailed Product Information

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