Setup of 3-D Life Hydrogels

Use this form to calculate the volumes of gel components required for the setup of 3-D Life Hydrogels of various compositions.
For detailed information please refer to the Hydrogel User Guide and protocols for each type of gel.

  1. In the first row enter the stock concentrations of your 3-D Life reagents. The standard stock concentrations are already shown.
  2. In the second row enter the final crosslinking concentration and the final concentrations of added peptides or Thioglycerol.
  3. In the third row enter the volume of your biological sample (e. g. cell suspension, tissue pieces) to be added and the total hydrogel volume.
  4. Click "Calculate volumes" to receive your pipetting protocol.
  5. Red bordered fields are mandatory to allow the calculation of a gel composition.

Stock concentrations:

Thiol-reactive polymer:
  mmol/L
Crosslinker:
  mmol/L
RGD Peptide:
  mmol/L
GFOGER-3 Peptide:
  mg/mL
Thioglycerol:
  mmol/L

Final Concentrations:

Crosslinking concentration:

  mmol/L

RGD Peptide:
  mmol/L
GFOGER-3 Peptide:
  mg/mL
Thioglycerol:
  mmol/L

Biological sample:

  µl

Final Volume:

  µl

 

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