m91_3_237995616

3-D Life SG-Dextran


Catalog Number: M91-3

Unit Size: 3x 170 µl

Shipping Temperature: Ambient


Price: 160,00 €

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  • Description
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Details
Supporting Information

Description 3-D Life SG-Dextran is a component of the 3-D Life Hydrogel system. It contains dextran functionalized with thiol-reactive groups (SG-Dextran) that can be used together with PEG-Link (Cat. No.L50-1 or L50-3) or CD-Link (Cat. No. L60-1 or L60-3) to set up slow gelling, cell-compatible hydrogels.

When crosslinked with PEG-Link or CD-Link Dextran-based 3-D Life Hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation. Dextran hydrogels crosslinked with HyLink cannot be dissolved by dextranase.

10 x CB (pH 7.2) is a buffer to control pH and osmotic conditions during gel formation in the presence of cells. For more information and instructions, please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.
Catalog Number M91-3
Application 3-D cell cultures; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.
Quantity Allows formation of up to 6 ml 3-D Life Hydrogel depending on the stiffness of the gel, if used together with thiol-functionalized polymers (3-D Life PEG-Link or CD-Link).
Components
Material Quantity Concentration
SG-Dextran3 x 170 µl30 mmol/L thioreactive groupsMSDS
10x CB (pH 7.2)600 µln.a.MSDS
Water4 x 1500 µln.aMSDS
Ambient
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