Cell Lines and Primary Cells used in 3-D Life Hydrogels
| Cell Type | Application | References |
|---|---|---|
| Primary Cells | ||
| Fibroblasts (human) | Tumor-stroma model | Kuehlbach et al., 2021 Hensler et al., 2021 |
| hMSCs (human mesenchymal stem cells) | Mechanotransduction | Wang et al., 2021 Benz, K., et al., 2010 |
| Chondrocytes | Tissue engineering; cell-based therapeutic development | Lang et al., 2021 Rothdiener M. et al., 2018 Benz, K., et al., 2010 |
| Pancreatic progenitor cells (human) | Organoid culture | Georgakopoulos et al., 2020 |
| Cardiomyocytes (murine) | Mechanotransduction | Friedrich et al., 2019 Friedrich, O. et al., 2017 |
| PBMCs (human peripheral blood mononuclear cells) | Lymph node model; perfusion | Kraus et al., 2019 |
| APCs (antigen presenting cells) DCs (dentritic cells) ATSVF (Adipose tissue stromal vascular fraction) BM-MSC (bone marrow mesenchymal stem cells) ADSCs (adipose-derived stem cells) UC-MSC (umbilical cord mesenchymal stem cells) | Lymph node model; perfusion | Sardi, M. et al., 2016 |
| EPCs (endothelial progenitor cells, murine) | Matrix-stiffness-dependent cell differentiation; hydrogel implantation in mice | Xue et al., 2019 |
| Neurospheres (human) | Neurotoxicity testing | Hellwig, C. et al., 2018 |
| Nucleus pulposus (human) | Mechanotransduction | He et al., 2018 |
| Intervertebral disk cells (human) | Cell-based therapeutic development | Benz, K., et al., 2010 |
| Pancreatic islets (murine) | Cell-based therapeutic development; implantation | Ayenehdeh et al., 2017 |
| AT-MSCs (adipose tissue derived mesenchymal stem cells, murine) | Cell-based therapeutic development; implantation | Ayenehdeh et al., 2017 |
| HUVECs (Human Umbilical Vein Endothelial Cells) | Cell-based therapeutic development; implantation; angiogenesis | Koenig, G., et al., 2016 Sun, J. et al., 2014 |
| ZHBTc4 ESCs (mouse embryonic stem cells) | Microsphere production, microsphere cultivation, scRNA-seq, fluorescent labeling of hydrogel via biotin-streptavidin attachment | Sun et al., 2021 Bavli et al., 2021 |
| PMN (human polymorphonuclear leukocytes, neutrophils, from whole blood) PBMCs (human peripheral blood mononucleated cells, from whole blood) primary fibroblasts (from human cervix uteri) combined with SiHa spheroids | Tumor-stroma model on microfluidic platform; drug testing | Kromidas et al., 2023 |
| Enteric neural stem cells, murine | Neurosphere outgrowth; nervous system networks | Chen et al., 2023 |
| HUVECs (Human Umbilical Vein Endothelial Cells) Hs27 (human foreskin fibroblasts) | Angiogenesis model | Hafa et al., 2023 |
| Human breast cancer organoids (patient derived) T cells, CAR-T cells (human) Fibroblasts (human) | Tumor-on-chip model including T and CAR-T cells investigating extravasation, tumor infiltration and cytokine release; drug testing | Maulana et al., 2024 |
| Human melanocytes | Microphysiological System (MPS), Organ-on-Chip, perfusion, drug screening, safety sceening | Cipriano et al., 2022 |
| murine pancreatic organoids | organoid culture | Jung et al., 2021 |
| human dermal fibroblasts | tumor-stroma model | Angres, B. and Wurst, H., 2017 |
| HSkMCs (primary human skeletal muscle cells) | injured skeletal muscle model | Yuan et al., 2024 Zhao et al., 2021 |
| Cell Lines | ||
| U937 (lymphoblast) HL-60 (promyelocytic) HaCaT (keratinocyte) MFC-7 (mamma carcinoma, human) H838 (lung carcinoma) | Tumor-stroma model | Hensler et al., 2021 |
| MCF-7 (mamma carcinoma, human), human dermal fibroblasts (primary) | Tumor-stroma model | Angres, B. and Wurst, H., 2017 |
| HT29 (colon adenocarcinoma, human) MDCK (kidney epithelial, canine) | Epithelial barrier model; microfluidics | Nair et al., 2021 |
| OCI-AML3 (leukemic) | Migration assay | Zippel et al., 2019 |
| HepG2 (hepatocyte carcinoma, human) | Spheroid culture | Miyakawa et al., 2018 |
| HepG2 (hepatocyte carcinoma, human) Huh-7 (hepatocyte carcinoma, human) | Mechanotransduction | Shen et al., 2019 |
| MCF10A (mammary breast epithelial, non tumorigenic, human) | Cancer research | Grobe, H. et al., 2018 Grikscheit, K. et al., 2015 |
| U87 (glioblastoma, human) | Cancer research, chemotaxis, cell migration | Huang et al., 2018 |
| HKC-8 (kidney proximal tubular, human) PU009-F (renal fibroblasts, human) | Kidney fibrosis model | Nugraha et al., 2017 |
| KCOT (keratocystic odontogenic tumor, human) | Cancer research, spheroid culture | Noguchi et al., 2017 |
| 3T3 (fibroblast, murine) | Tisssue engineering | Koenig, G., et al., 2016 |
| HCT-116 (colon carcinoma, human) | Drug screening | Kuehlbach et al., 2021 Rimann, M., Graf-Hausner, U., 2012 |
| HeLa (adenocarcinoma, human) HT1080 (epithelial cancer cell line from fibrosarcoma, human) MDA-MB-231 (mamma carcinoma, human) NES (neuroepithelial-like stem cell derived from H9 hESC, human) | Microarray | Ueda, E., et al., 2012 |
| C2C12 (myoblasts, murine) | Cell-matrix interactions; mechanotransduction | Yin et al., 2021 Wang et al., 2019 |
| PC9 (lung adenocarcinoma, human) | Microsphere production, microsphere cultivation, scRNA-seq, fluorescent labeling of hydrogel via biotin-streptavidin attachment | Sun et al., 2021 Bavli et al., 2021 |
| SiHa (human squamous cervical cancer cell line) | Tumor-stroma model on microfluidic platform; drug testing; tumor-on-chip | Kromidas et al., 2023 |
| Melanoma cell lines | Migration assay | Militaru et al., 2023 |
| HaCaT (human keratinocytes) Hs27 (human foreskin fibroblasts) | Full-thickness skin model | Hafa et al., 2023 |
| MDA-MB-231 (human breast cancer cell line) | Tumor-on-chip model including T and CAR-T cells investigating extravasation, tumor infiltration and cytokine release; drug testing | Maulana et al., 2024 |
| HeLa Kyoto (adenocarcinoma, human) | Investigation of interaction of cells with precisely immobilized proteins or peptides in 3D cell cultures. | Krüger et al., 2022 |
| WM1346 (human melanoma) WM1366 (human melanoma) Malme3M (human melanoma) A375 (human melanoma) | drug testing, spheroid culture | Del Mistro et al., 2022 |
| 451LU (human melanoma) | drug testing, cancer research, spheroid culture | Albrecht et al., 2022 |
| transgenic mouse pancreas organoid cell line | organoid culture | Jung et al., 2021 |
| H838 (human non-small-cell lung carcinoma) | Tumor-Stroma model | Hensler et al., 2021 |
| HKC-8 (kidney proximal tubular, human) | Kidney fibrosis model | Palau et al., 2021 Nugraha et al., 2017 |
| PU009-F (renal fibroblasts, human) | Kidney fibrosis model | Palau et al., 2021 Nugraha et al., 2017 |