3-D Life Hydrogels: Cells and Applications
| Cell Type | Application | References |
|---|---|---|
| Primary Cells | ||
| Fibroblasts (human) | Tumor-on-chip model, tumor infiltration, cell extravasation, drug screening, drug testing, cytokine release | Maulana et al., 2024 |
| hMSCs (human mesenchymal stem cells) | Mechanotransduction | Wang et al., 2021 |
| Chondrocytes | Chondrogenesis, tissue regeneration, tissue engineering | Lang et al., 2021 Rothdiener M. et al., 2018 Benz, K., et al., 2010 |
| Pancreatic progenitor cells (human) | Organoid culture | Georgakopoulos et al., 2020 |
| Cardiomyocytes (murine) | Mechanotransduction, cardiac research | Friedrich et al., 2019 Friedrich, O. et al., 2017 |
| PBMCs (human peripheral blood mononuclear cells) | Lymph node model | Kraus et al., 2019 Sardi, M. et al., 2016 |
| EPCs (endothelial progenitor cells, murine) | Vasculogenesis, angiogenesis | Xue et al., 2019 |
| Neurospheres (human) | Neurotoxicity testing | Hellwig, C. et al., 2018 |
| Nucleus pulposus cells (human) | Cartilage research | He et al., 2018 |
| Intervertebral disk cells (human) | Chondrogenesis, tissue regeneration | Benz, K., et al., 2010 |
| Pancreatic islets (murine) | Diabetes research, islet transplantation | Ayenehdeh et al., 2017 |
| AT-MSCs (adipose tissue derived mesenchymal stem cells, murine) | Diabetes research, islet transplantation | Ayenehdeh et al., 2017 |
| HUVECs (Human Umbilical Vein Endothelial Cells) | Cell-based therapeutic development, tumor-stroma model, drug screening, cancer research | Kuehlbach et al., 2021 Sun, J. et al., 2014 |
| ZHBTc4 ESCs (mouse embryonic stem cells) | Microsphere production, microsphere cultivation, scRNA-seq, fluorescent labeling of hydrogel via biotin-streptavidin attachment | Sun et al., 2021 Bavli et al., 2021 |
| PMNs (human polymorphonuclear leukocytes, neutrophils, from whole blood) | Tumor-stroma model on microfluidic platform; drug testing | Kromidas et al., 2023 |
| Enteric neural stem cells (murine) | Neurosphere outgrowth; nervous system networks | Chen et al., 2023 |
| HUVECs (Human Umbilical Vein Endothelial Cells) | Angiogenesis | |
| Human breast cancer organoids (patient derived) | Tumor-on-chip model, tumor infiltration, cell extravasation, drug screening, drug testing, cytokine release | Maulana et al., 2024 |
| Human melanocytes | Microphysiological system (MPS), Organ-on-Chip, perfusion, drug screening, safety sceening, choroid-on-chip | Cipriano et al., 2022 |
| Pancreatic organoids (murine) | Organoid culture | Jung et al., 2021 |
| Human dermal fibroblasts | Tumor-stroma model, drug screening, cell-based therapeutic development, drug testing, cancer research | Kuehlbach et al., 2021 Angres, B. and Wurst, H., 2017 |
| HSkMCs (primary human skeletal muscle cells) | Injured skeletal muscle model | Yuan et al., 2024 Zhao et al., 2021 |
| T cells | Microphysiological System (MPS), Organ-on-Chip, perfusion, drug screening, safety sceening, Choroid-on-Chip, tumor-on-chip model, tumor infiltration, cell extravasation, drug testing, cytokine release | Maulana et al., 2024 Cipriano et al., 2022 |
| PBMCs (human peripheral blood mononucleated cells, from whole blood) | Lymph node model, tumor-stroma model, cancer-on-chip-model, organ-on-chip model, long-term culture, microphysiological systems (MPS) | |
| Fibroblasts (from human cervix uteri) | Tumor-stroma model, cancer-on-chip-model, organ-on-chip model, long-term culture, microphysiological systems (MPS) | Kromidas et al., 2023 |
| Monocytes | Lymph node model | Kraus et al., 2019 |
| Dendritic cells (DC) | Lymph node model | Kraus et al., 2019 |
| Adipose tissue stromal vascular fraction cells | Lymph node model | Kraus et al., 2019 |
| Human chondrocytes | Chondrogenesis, tissue regeneration, implantation, angiogenesis, cell invasion barrier, biocompatibility studies, cartilage research | Rothdiener M. et al., 2018 Scholz, B., et al., 2010 Benz, K., et al., 2010 |
| APCs (antigen presenting cells) | Lymph node model | Sardi, M. et al., 2016 |
| ATSVF (Adipose tissue stromal vascular fraction) | Lymph node model | Sardi, M. et al., 2016 |
| BM-MSC (bone marrow mesenchymal stem cells) | Lymph node model | Sardi, M. et al., 2016 |
| ADSCs (adipose-derived stem cells) | Lymph node model | Sardi, M. et al., 2016 |
| UC-MSC (umbilical cord mesenchymal stem cells) | Lymph node model | Sardi, M. et al., 2016 |
| Invertebral disc chondrocytes | Chondrogenesis, tissue regeneration, cell invasion barrier | Scholz, B., et al., 2010 |
| HUVECs (Human Umbilical Vein Endothelial Cells) | Implantation, angiogenesis, cell invasion barrier, chondrogenesis, tissue regeneration, biocompatibility studies, cartilage research | Scholz, B., et al., 2010 |
| PBMCs (human peripheral blood mononuclear cells) | Tumor-stroma model, cancer-on-chip-model, Organ-on-Chip model, long-term culture, microphysiological systems (MPS) | Kromidas et al., 2023 |
| PBMCs (human peripheral blood mononuclear cells) | Microphysiological system (MPS), Organ-on-Chip, perfusion, drug screening, safety sceening, Choroid-on-Chip | Cipriano et al., 2022 |
| Chondrocytes | Implantation, angiogenesis, cell invasion barrier, chondrogenesis, tissue regeneration, biocompatibility studies, cartilage research | Scholz, B., et al., 2010 |
| hMSCs (human mesenchymal stem cells) | Chondrogenesis, tissue regeneration | Benz, K., et al., 2010 |
| Human dermal fibroblasts | Cell metabolism studies | Charwat, V. et al., 2015 |
| Human melanoma cells, metastatic | spheroid culture, cancer research, drug testing | Dunsche et al., 2023 |
| chronic lymphocyte leukemia (CLL) cells (human) | personalized medicine, Differential Gene Expression Analysis (DGEA), functional precision oncology | El Faraj et al., 2024 |
| Cell Lines | ||
| U937 (lymphoblast) HL-60 (promyelocytic) HaCaT (keratinocyte) MFC-7 (mamma carcinoma, human) H838 (lung carcinoma) | Tumor-stroma model | |
| MCF-7 (mamma carcinoma, human) | Tumor-stroma model, lymph node model, cell metabolism studies | Salarian et al., 2024 Hensler et al., 2021 Angres, B. and Wurst, H., 2017 Sardi, M. et al., 2016 Charwat, V. et al., 2015 |
| HT29 (colon adenocarcinoma, human) | Epithelial barrier model, microfluidics, transepithelial cell migration | Nair et al., 2021 |
| OCI-AML3 (leukemic) | Migration assay, leukemia research | Zippel et al., 2019 |
| HepG2 (hepatocyte carcinoma, human) | Liver cancer research | Shen et al., 2019 |
| HepG2 (hepatocyte carcinoma, human) | Hepatitis research, drug testing, spheroid culture | Miyakawa et al., 2018 |
| MCF-10A (mammary breast epithelial, non tumorigenic, human) | Epithelial junction formation, epithelial development, epithelial lumen formation | Grobe, H. et al., 2018 Grikscheit, K. et al., 2015 |
| U87 (glioblastoma, human) | Cancer research, chemotaxis, cell migration | Huang et al., 2018 |
| HKC-8 (kidney proximal tubular, human) | Kidney fibrosis model | Palau et al., 2021 Nugraha et al., 2017 |
| KCOT (keratocystic odontogenic tumor, human) | Cancer research, spheroid culture | Noguchi et al., 2017 |
| 3T3 (fibroblast, murine) | Tisssue engineering, hydrogel tailoring, collective cell behavior | Wang et al., 2019 Koenig, G., et al., 2016 |
| HCT 116 (colon carcinoma, human) | Drug screening, automation | Rimann, M. et al., 2014 |
| HeLa (adenocarcinoma, human) HT1080 (epithelial cancer cell line from fibrosarcoma, human) MDA-MB-231 (mamma carcinoma, human) NES (neuroepithelial-like stem cell derived from H9 hESC, human) | High-throughput (HT) cell screening, drug screening, cell mircoarray development, cytotoxicity screening | |
| C2C12 (myoblasts, murine) | Cell-matrix interactions, mechanotransduction, collective cell behavior, hydrogel tailoring | Yin et al., 2021 Wang et al., 2019 |
| PC9 (lung adenocarcinoma, human) | Microsphere production, microsphere cultivation, scRNA-seq, fluorescent labeling of hydrogel via biotin-streptavidin attachment | Sun et al., 2021 Bavli et al., 2021 |
| SiHa (human squamous cervical cancer cell line) | Tumor-stroma model on microfluidic platform, drug testing; tumor-on-chip | Kromidas et al., 2023 |
| Melanoma cell lines | Migration assay | Militaru et al., 2023 |
| HaCaT (human keratinocytes) | Full-thickness skin model; tumor-stroma model, drug testing | |
| MDA-MB-231 (human breast cancer cell line) | Tumor-on-chip model, tumor infiltration, cell extravasation, drug screening, drug testing, cytokine release, high-throughput (HT) cell screening, drug screening, automation, cell mircoarray development, cytotoxicity screening | Maulana et al., 2024 Ueda, E., et al., 2012 |
| HeLa Kyoto (adenocarcinoma, human) | 4D hydrogel, photoresponsive hydrogels, photoinstructive hydrogels | Krüger et al., 2022 |
| WM1346 (human melanoma) WM1366 (human melanoma) Malme3M (human melanoma) A375 (human melanoma) | Drug testing, spheroid culture | Del Mistro et al., 2022 |
| 451LU (human melanoma) | Drug testing, cancer research, spheroid culture | Albrecht et al., 2022 |
| Transgenic mouse pancreas organoid cell line | Pancreatic organoid culture | |
| H838 (human non-small-cell lung carcinoma) | Tumor-Stroma model, drug screening, cell-based therapeutic development, drug testing, cancer research | Kuehlbach et al., 2021 Hensler et al., 2021 |
| HKC-8 (kidney proximal tubular, human) | Kidney fibrosis model | |
| PU009-F (renal fibroblasts, human) | Kidney fibrosis model | Nugraha et al., 2017 |
| U937 (human lymphoblast) | Tumor-stroma model, drug screening, cell-based therapeutic development, drug testing, cancer research | Kuehlbach et al., 2021 Hensler et al., 2021 |
| HL-60 (human, differentiated to neutrophils) | Tumor-stroma model, drug screening, cell-based therapeutic development, drug testing, cancer research | Kuehlbach et al., 2021 Hensler et al., 2021 |
| HCT 116 (colon carcinoma, human) | Drug screening, tumor-stroma model, cell-based therapeutic development, drug testing, Cancer research | Kuehlbach et al., 2021 |
| Huh-7 (hepatic cancer) | Liver cancer research | Shen et al., 2019 |
| HaCaT (human keratinocytes) | Tumor-stroma model, drug testing, full-thickness skin model, angiogenesis model | Hafa et al., 2023 Hensler et al., 2021 |
| Hs27 (human foreskin fibroblasts) | Full-thickness skin model, angiogenesis model | Hafa et al., 2023 |
| MDCK (kidney epithelial, canine) | Cell-based assays in microfluidic platforms, in vitro model of cellular barriers, transepithelial cell migration | Nair et al., 2021 |
| No cells used | Immobilization of DNA in hydrogels, 3D-light scattering biosensing | Rothbauer et al., 2020 Weyel et al., 2017 |
| HT1080 (epithelial cancer cell line from fibrosarcoma, human) | Microarray | Ueda, E., et al., 2012 |
| HeLa (adenocarcinoma, human) | Microarray | Ueda, E., et al., 2012 |
| NES (neuroepithelial-like stem cell derived from H9 hESC, human) | Microarray | Ueda, E., et al., 2012 |
| HUVECs (Human Umbilical Vein Endothelial Cells) | Implantation, biocompatibility, titanium-hydrogel implant development | Koenig, G., et al., 2016 |
| Jurkat T-cells (human lymphocytes) | microarray, spheroid culture, High-Throuput Screening (HTS) | Salarian et al., 2024 |
| CAR-T cells | Tumor-on-chip model, tumor infiltration, cell extravasation, drug screening, drug testing, cytokine release | Maulana et al., 2024 |
| Prokaryotes | ||
| Staphylococcus aureus | In gel immobilization | Neugebauer, U., et al., 2012 |