3-D Life ToGro Hydrogel

Catalog Number: G94-1

Unit Size: 1 Kit

Shipping Temperature: Ambient

286,00 €

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  • Description
  • Specifications

Details
Supporting Information

Description The 3-D Life ToGro Hydrogel Kit contains reagents for the preparation of hydrogels with a soft stiffness (400 Pa; shear modulus) and a RGD Peptide concentration of 0.4 mmol/L. The gel composition allows three-dimensional spreading and migration of most cells. The gel is formed by the crosslinking of modified dextran carrying the cell adhesion motif RGD (RGD-Dextran) with CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with thiol-reactive groups on RGD-Dextran resulting in gel formation within 20 min. The formation of hydrogel is performed at physiological pH for optimal cell compatibility.

CD-Link contains a matrix metalloproteinase (MMP)-cleavable peptide sequence (Pro-Leu-Gly-Leu-Trp-Ala), which is cleaved by a broad range of MMPs including MMP1, MMP2, MMP3, MMP7, MMP9 and MMP13 [1, 2, 3]. It allows cells to locally degrade the polymer network, if they produce the indicated MMPs.

3-D Life ToGro Hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase Cat. No. D10-1), which allows recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or further cultivation.

For preparation of gels follow the instructions of the 3-D Life ToGro Protocol GP-3 “Preparation of 3-D Life ToGro Hydrogel”. The document can be downloaded.
Catalog Number G94-1
Application 3-dimensional cultivation of various cell types, spheroids, tissue samples and similar requiring a cell-adhesive and cell-degradable matrix for cell growth and differentiation.
Quantity Allows formation of 2.4 ml 3-D Life ToGro Hydrogel.
Components
Material Quantity Concentration
RGD-Dextran, lyophilized2 x 870 µl*n.a.
CD-Link, lyophilized200 µl*20 mmol/L* thiol groups
Reconstitution Buffer2 x 900 µln.a
Water600 µln/a
*Volume/concentration after reconstitution of lyophilisate.
Reference[1] Knight, C. G. et al. FEBS 296:263-66 (1992) [2] Patterson, J. and Hubbell, J. A. Biomaterials 31, 7836-7845 (2010) [3] Deng, S. et al. J. Biol. Chem. 275, No. 40, 31422–31427 (2000)

General Protocols

Preconfigured ToGro Hydrogel

Data Sheet

Detailed Product Information

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