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3-D Life
PVA-HA Hydrogel
Catalog Number: G85-1
Unit Size: 1 Kit
Shipping Temperature: Ambient
Price:
194,00 €
How to Order
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Description
Specifications
Details
Supporting Information
Description
The
3-D Life
PVA-HA Hydrogel Kit provides reagents for setting up hydrogels containing hyaluronic acid. Its major components are SG-PVA and the thiol-functionalized hyaluronic acid crosslinker HyLink. When the two reagents are combined, thiol groups on HyLink form stable thioether bonds with thiol-reactive groups on SG-PVA, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation. The hydrogel allows cell spreading and migration if cell adhesion peptides are present in the gel.Prior to the crosslinking step, cell adhesion peptides (e.g.
3-D Life
RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups on SG-PVA to provide a cell-adhesive matrix. For more information and instructions, please consult the General Protocol GP-4 "Preparation of
3-D Life
Hyaluronic Acid (HA) Hydrogels" and the
3-D Life
Hydrogels User Guide.
Catalog Number
G85-1
Application
3-D cell culture, hydrogel injections, filling of microchannels, generation of soft to very stiff gels .
Quantity
Allows formation of 3.3 ml
3-D Life
Hydrogel of a crosslinking strength of 1.2 mmol/L.
Components
Material
Quantity
Concentration
SG-PVA
170 µl
30 mmol/L thioreactive groups
MSDS
HyLink, lyophilized
2 x 200 µl*
10 mmol/L* thiol groups
MSDS
10x CB (pH 7.2)
200 µl
n.a.
MSDS
Water
2 x 1500 µl
n.a
MSDS
*Volume/concentration after reconstitution of lyophilisate.
General Protocols
Hyaluronic acid hydrogels
User Guide
3-D Life
Hydrogel User Guide
Data Sheet
Detailed Product Information
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