Cell environment design at your fingertips

Cell environment design at your fingertips


Fully customizable and easy to use

Hydrogels free of animal-derived components

Hydrogels free
of animal-derived components


Biomimetic modifications

Biomimetic modifications



Control cell adhesion


Include Matrix Metalloprotease (MMP) functions

3-D Life Biomimetic Hydrogels
Cell Environment Design at Your Fingertips

Cellendes is a life science company offering comprehensive solutions for controlled design of cell environments in 3-D cell culture. A toolbox of reagents provides flexibility in composing biomimetic hydrogels right at the users bench. Areas of application include basic research, drug development and biomedical engineering.

Technology

Technology

Our 3-D Life technology is an integrated system for the generation of biomimetic hydrogels for 3-D cell culture. The 3-D Life system consists of two polymers that form a hydrogel by covalent crosslinking at ambient temperature.
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Applications & Protocols

Applications & Protocols

Applications and detailed protocols illustrate the use of the 3-D Life technology.




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Products

Products

The 3-D Life product line represents the most flexible hydrogel system for biomimetic hydrogel design, from pre-configured gels to single components for individual gel compositions.

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Popular Products

3-D Life ToGro Hydrogel
2024-01-23 11:28
G94-1
1 Kit
Ambient
Price: 279,00 €
Add to cart
3-D Life Dextran-PEG Hydrogel SG
2024-01-24 14:44
G92-1
1 Kit
Ambient
Price: 89,00 €
Add to cart
3-D Life Dextran-CD Hydrogel SG
2024-01-24 14:50
G93-1
1 Kit
Ambient
Price: 200,00 €
Add to cart
3-D Life PVA-PEG Hydrogel SG
2024-01-24 10:19
G82-1
1 Kit
Ambient
Price: 83,00 €
Add to cart
fg93_1
Cell Degradable

3-D Life Dextran-CD Hydrogel SG

Catalog Number: G93-1

Unit Size: 1 Kit

Shipping Temperature: Ambient

Price: 200,00 €

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How to Order
To order products directly from Cellendes please
  • use the Online Shop
  • email us
  • or call us at +49-7121-15940-0.

Please take notice of our shipping charges.

Cellendes products are also supplied by international distribution partners.

  • Description
  • Specifications

Details
Supporting Information

Description The 3-D Life Dextran-CD Hydrogel SG Kit provides reagents for setting up slow gelling, cell-compatible hydrogels. Its major components are SG-Dextran and the crosslinker CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with thiol-reactive groups on SG-Dextran, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation.

CD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala). The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9 [1]. It allows cells to spread and migrate within the hydrogel if they express the indicated MMPs. In most cases cell spreading and migration also requires the presence of adhesion peptides.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups on SG-Dextran to provide a cell-adhesive matrix.

SG-Dextran hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

For instructions, please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.
Catalog Number G93-1
Application 3-D cell culture, hydrogel injections, filling of microchannels, generation of soft to very stiff gels.
Quantity Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.
Components
Material Quantity Concentration
SG-Dextran170 µl30 mmol/L thioreactive groupsMSDS
CD-Link, lyophilized200 µl*20 mmol/L* thiol groupsMSDS
10x CB (pH 7.2)200 µln.a.MSDS
Water2 x 1500 µln.aMSDS
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

Slow-gelling hydrogels

User Guide

3-D Life Hydrogel User Guide

Data Sheet

Detailed Product Information

Ambient

What our customers say

Dr. Katharina Grikscheit
University of Frankfurt, Germany
3-D Life Hydrogels were especially suited for long term epithelial cultures with an easy to optimize system and high yield of spheroid formation.

Dr. Pavel Levkin
Karlsruhe Inst. of Technology, Germany
The 3-D Life Hydrogel was especially appealing because it is a facile and quick method, gentle in terms of cell handling, and does not require exposing cells to UV light.
Dr. Cornelia Lee-Thedieck
Leibniz University Hannover, Germany
Dextran-PEG Hydrogel offers an easy and reliable platform for culturing cells in 3D.



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