g95_1
Hyaluronic Acid

3-D Life Dextran-HA Hydrogel

Catalog Number: G95-1

Unit Size: 1 Kit

Shipping Temperature: Ambient

Price: 200,00 €

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  • Description
  • Specifications

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Supporting Information

Description The 3-D Life Dextran-HA Hydrogel Kit provides reagents for setting up hydrogels containing hyaluronic acid. Its major components are SG-Dextran and the thiol-functionalized hyaluronic acid crosslinker HyLink. When SG-Dextran and HyLink are combined, thiol groups on HyLink form stable thioether bonds with thiol-reactive groups on SG-Dextran, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation. The hydrogel allows cell spreading and migration if cell adhesion peptides are present in the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups on SG-Dextran to provide a cell-adhesive matrix.

Dextran hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

For more information and instructions, please consult the General Protocol GP-4 "Preparation of 3-D Life Hyaluronic Acid (HA) Hydrogels" and the 3-D Life Hydrogels User Guide.
Catalog Number G95-1
Application 3-D cell culture, hydrogel injections, filling of microchannels, generation of soft to very stiff gels.
Quantity Allows formation of 3.3 ml 3-D Life Hydrogel of a crosslinking strength of 1.2 mmol/L.
Components
Material Quantity Concentration
SG-Dextran170 µl30 mmol/L thioreactive groupsMSDS
HyLink, lyophilized2 x 200 µl*10 mmol/L* thiol groupsMSDS
10x CB (pH 7.2)200 µln.a.MSDS
Water2 x 1500 µln.aMSDS
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

Hyaluronic acid hydrogels

User Guide

3-D Life Hydrogel User Guide

Data Sheet

Detailed Product Information

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