3-D Life PVA-PEG Hydrogel FG

Catalog Number: FG80-1

Unit Size: 1 Kit

Shipping Temperature: Dry Ice

Price: 83,00 €

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Description
Specifications

Details

Supporting Information

Description

The 3-D Life PVA-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized polyvinyl alcohol (Mal-PVA) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on Mal-PVA, which results in the formation of the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-PVA to provide a cell-adhesive matrix.

For more information and instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

FG80-1

Application

3-D cell cultures, hydrogel barriers.

Quantity

Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.

Components

Material	Quantity	Concentration
Mal-PVA	170 µl	30 mmol/L maleimide groups	MSDS
PEG-Link, lyophilized	200 µl*	20 mmol/L* thiol groups	MSDS
10x CB (pH 5.5)	200 µl	n.a	MSDS
Water	2 x 1500 µl	n.a	MSDS
*Volume/concentration after reconstitution of lyophilisate.

3-D Life PVA-PEG Hydrogel FG

General Protocols

Applications

User Guide

Data Sheet

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