Cell environment design at your fingertips

Cell environment design at your fingertips


Fully customizable and easy to use

Hydrogels free of animal-derived components

Hydrogels free
of animal-derived components


Biomimetic modifications

Biomimetic modifications



Control cell adhesion


Include Matrix Metalloprotease (MMP) functions

3-D Life Biomimetic Hydrogels
Cell Environment Design at Your Fingertips

Cellendes is a life science company offering comprehensive solutions for controlled design of cell environments in 3-D cell culture. A toolbox of reagents provides flexibility in composing biomimetic hydrogels right at the users bench. Areas of application include basic research, drug development and biomedical engineering.

Technology

Technology

Our 3-D Life technology is an integrated system for the generation of biomimetic hydrogels for 3-D cell culture. The 3-D Life system consists of two polymers that form a hydrogel by covalent crosslinking at ambient temperature.
Read More
Applications & Protocols

Applications & Protocols

Applications and detailed protocols illustrate the use of the 3-D Life technology.




Read More
Products

Products

The 3-D Life product line represents the most flexible hydrogel system for biomimetic hydrogel design, from pre-configured gels to single components for individual gel compositions.

Read More

Popular Products

3-D Life ToGro Hydrogel
2024-01-23 11:28
G94-1
1 Kit
Ambient
Price: 279,00 €
Add to cart
3-D Life Dextran-PEG Hydrogel SG
2024-01-24 14:44
G92-1
1 Kit
Ambient
Price: 89,00 €
Add to cart
3-D Life Dextran-CD Hydrogel SG
2024-01-24 14:50
G93-1
1 Kit
Ambient
Price: 200,00 €
Add to cart
3-D Life PVA-PEG Hydrogel SG
2024-01-24 10:19
G82-1
1 Kit
Ambient
Price: 83,00 €
Add to cart
g94_1_aa
Cell Degradable

3-D Life ToGro Hydrogel

Catalog Number: G94-1

Unit Size: 1 Kit

Shipping Temperature: Ambient

Price: 279,00 €

Add to cart
How to Order
To order products directly from Cellendes please
  • use the Online Shop
  • email us
  • or call us at +49-7121-15940-0.

Please take notice of our shipping charges.

Cellendes products are also supplied by international distribution partners.

  • Description
  • Specifications

Details
Supporting Information

Description The 3-D Life ToGro Hydrogel Kit contains reagents for the preparation of hydrogels with a soft stiffness (400 Pa; shear modulus) and a RGD Peptide concentration of 0.4 mmol/L. The gel composition allows three-dimensional spreading and migration of most cells. The gel is formed by the crosslinking of modified dextran carrying the cell adhesion motif RGD (RGD-Dextran) with CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with thiol-reactive groups on RGD-Dextran resulting in gel formation within 20 min. The formation of hydrogel is performed at physiological pH for optimal cell compatibility. CD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide. The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9 [1]. It allows cells to spread and migrate within the gel if they express the indicated MMPs. 3-D Life ToGro hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase Cat. No. D10-1), which allows recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or further cultivation. For preparation of gels follow the instructions of the 3-D Life ToGro Protocol GP-3 “Preparation of 3-D Life ToGro Hydrogel”. The document can be downloaded.
Catalog Number G94-1
Application 3-dimensional cultivation of various cell types, spheroids, tissue samples and similar requiring a cell-adhesive and cell-degradable matrix for cell growth and differentiation.
Quantity Allows formation of 2.4 ml 3-D Life ToGro Hydrogel.
Components
Material Quantity Concentration
RGD-Dextran, lyophilized2 x 870 µl*n.a.MSDS
CD-Link, lyophilized200 µl*20 mmol/L* thiol groupsMSDS
Reconstitution Buffer2 x 900 µln.aMSDS
Water600 µln/aMSDS
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

Preconfigured ToGro Hydrogel

Data Sheet

Detailed Product Information

Ambient

What our customers say

Dr. Katharina Grikscheit
University of Frankfurt, Germany
3-D Life Hydrogels were especially suited for long term epithelial cultures with an easy to optimize system and high yield of spheroid formation.

Dr. Pavel Levkin
Karlsruhe Inst. of Technology, Germany
The 3-D Life Hydrogel was especially appealing because it is a facile and quick method, gentle in terms of cell handling, and does not require exposing cells to UV light.
Dr. Cornelia Lee-Thedieck
Leibniz University Hannover, Germany
Dextran-PEG Hydrogel offers an easy and reliable platform for culturing cells in 3D.



Follow us
Company
Newsletter
Information
keyboard_arrow_up