3-D Life PVA-CD Hydrogel FG

Catalog Number: FG81-1

Unit Size: 1 Kit

Shipping Temperature: Dry Ice

199,00 €

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  • Description
  • Specifications

Details
Supporting Information

Description The 3-D Life PVA-CD Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized polyvinyl aclcohol (Mal-PVA) and the crosslinker CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with maleimide groups on Mal-PVA, which results in the formation of the gel.

CD-Link contains a matrix metalloproteinase (MMP)-cleavable peptide sequence (Pro-Leu-Gly-Leu-Trp-Ala), which is cleaved by a broad range of MMPs including MMP1, MMP2, MMP3, MMP7, MMP9 and MMP13 [1, 2, 3]. It allows cells to locally degrade the polymer network, if they produce the indicated MMPs.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat.No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-PVA to provide a cell-adhesive matrix.

For more information and instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.
Catalog Number FG81-1
Application 3-D cell culture
Quantity Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.
Components
Material Quantity Concentration
Mal-PVA170 µl30 mmol/L maleimide groups
CD-Link, lyophilized200 µl*20 mmol/L* thiol groups
10x CB (pH 5.5)200 µln.a
Water2 x 1500 µln.a
*Volume/concentration after reconstitution of lyophilisate.
Reference[1] Knight, C. G. et al. FEBS 296:263-66 (1992) [2] Patterson, J. and Hubbell, J. A. Biomaterials 31, 7836-7845 (2010) [3] Deng, S. et al. J. Biol. Chem. 275, No. 40, 31422–31427 (2000)

General Protocols

Fast-gelling Hydrogels

User Guide

3-D Life Hydrogel User Guide

Data Sheet

Detailed Product Information

Dry ice
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