3-D Life SG-Dextran
3-D Life SG-Dextran is a component of the 3-D Life Hydrogel system. It contains thiol-reactive dextran (SG-Dextran) that can be used together with PEG-Link (Cat. No.L50-1 or L50-3) or CD-Link (Cat. No. L60-1 or L60-3) to set up slow-gelling biomimetic hydrogels for 3-D cell culture.
|Description||3-D Life SG-Dextran is a component of the 3-D Life Hydrogel system. It contains dextran functionalized with thiol-reactive groups (SG-Dextran) that can be used together with PEG-Link (Cat. No.L50-1 or L50-3) or CD-Link (Cat. No. L60-1 or L60-3) to set up slow gelling, cell-compatible hydrogels. Dextran-based 3-D Life hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation. 10 x CB (pH 7.2) is a buffer to control pH and osmotic conditions during gel formation in the presence of cells. For more information and instructions, please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.|
|Application||3-D cell cultures; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.|
|Quantity||Allows formation of up to 6 ml 3-D Life Hydrogel depending on the stiffness of the gel, if used together with thiol-functionalized polymers (3-D Life PEG-Link or CD-Link).|