| Description |
3-D Life SG-Dextran is a component of the 3-D Life Hydrogel system. It
contains dextran functionalized with thiol-reactive groups (SG-Dextran) that
can be used together with PEG-Link (Cat. No.L50-1 or L50-3) or CD-Link (Cat.
No. L60-1 or L60-3) to set up slow gelling, cell-compatible hydrogels.
Dextran-based 3-D Life hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery
of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for
further cultivation.
10 x CB (pH 7.2) is a buffer to control pH and osmotic conditions during gel
formation in the presence of cells. For more information and instructions,
please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide. |
| Application |
3-D cell cultures; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution. |
| Quantity |
Allows formation of up to 6 ml 3-D Life Hydrogel depending on the stiffness of the gel, if used together with thiol-functionalized polymers (3-D Life PEG-Link or CD-Link). |
| Components |
| Material
|
Quantity
|
Concentration of reactive groups
|
|
| SG-Dextran | 3 x 170 µl | 30 mmol/L | MSDS | | 10x CB (pH 7.2) | 600 µl | n.a. | MSDS | | Water | 4 x 1500 µl | n.a | MSDS |
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