The 3-D Life PVA-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized polyvinyl alcohol (Mal-PVA) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on Mal-PVA, which results in the formation of the gel. Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-PVA to provide a cell-adhesive matrix.For more information and instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.
3-D cell cultures, hydrogel barriers.
Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.
|| Concentration of reactive groups
|Mal-PVA||170 µl||30 mmol/L||MSDS|
|PEG-Link, lyophilized||200 µl*||20 mmol/L||MSDS|
|10 x CB (pH 5.5)||200 µl||n.a||MSDS|
|Water||2 x 1500 µl||n.a||MSDS|
|*Volume/concentration after reconstitution of lyophilisate.