<em>3-D Life</em> PVA-PEG Hydrogel FG

3-D Life PVA-PEG Hydrogel FG

‎€70.00
SKU : FG80-1

Cat.No. : FG80-1


The 3-D Life PVA-PEG Hydrogel FG kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are polymeric Mal-PVA and PEG-Link.





Description The 3-D Life PVA-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized polyvinyl alcohol (Mal-PVA) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on Mal-PVA, which results in the formation of the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-PVA to provide a cell-adhesive matrix.

For more information and instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.
Application 3-D cell cultures, hydrogel barriers.
Quantity Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.
Components
Material Quantity Concentration
Mal-PVA170 µl30 mmol/LMSDS
PEG-Link, lyophilized200 µl*20 mmol/LMSDS
10 x CB (pH 5.5)200 µln.aMSDS
Water2 x 1500 µln.aMSDS
*Volume/concentration after reconstitution of lyophilisate.