3-D Life FG-Dextran is a component of the 3-D Life Hydrogel system. It
contains maleimide-functionalized dextran (Mal-Dextran) that can be used
together with PEG-Link (Cat. No.L50-1 or L50-3) or CD-Link (Cat. No. L60-1
or L60-3) to set up fast gelling, cell-compatible hydrogels.
Dextran-based 3-D Life hydrogels can be dissolved by the addition of
dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery
of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for
10 x CB (pH 7.2) is a buffer to control pH and osmotic conditions during gel
formation in the presence of cells. For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.
3-D cell cultures; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.
Allows formation of up to 6 ml 3-D Life Hydrogel depending on the stiffness of the gel, if used together with thiol-functionalized polymers (3-D Life PEG-Link or CD-Link).
|| Concentration of reactive groups
|Mal-Dextran, lyophilized||3 x 170 µl*||30 mmol/L||MSDS|
|10 x CB (pH 5.5)||600 µl||n.a.||MSDS|
|Water||4 x 1500 µl||n.a||MSDS|
|*Volume/concentration after reconstitution of lyophilisate.