Cell Lines and Primary Cells used in 3-D Life Hydrogels



Cell Type Application References
Cell Lines
U937 (lymphoblast)
HL-60 (promyelocytic)
HaCaT (keratinocyte)
MFC-7 (mamma carcinoma, human)
H838 (lung carcinoma)
Tumor-stroma modelHensler et al., 2021
MCF-7 (mamma carcinoma, human)
3T3 (fibroblasts, murine)
Tumor-stroma modelWang et al., 2021
Charwat, V. et al., 2015
HT29 (colon adenocarcinoma, human)
MDCK (kidney epithelial, canine)
Epithelial barrier model; microfluidicsNair et al., 2021
OCI-AML3 (leukemic)Migration assayZippel et al., 2019
HepG2 (hepatocyte carcinoma, human)Spheroid cultureMiyakawa et al., 2018
HepG2 (hepatocyte carcinoma, human)
Huh-7 (hepatocyte carcinoma, human)
MechanotransductionShen et al., 2019
MCF10A (mammary breast epithelial, non tumorigenic, human)Cancer researchGrobe, H. et al., 2018
Grikscheit, K. et al., 2015
U87 (glioblastoma, human)Cancer research, chemotaxis, cell migrationHuang et al., 2018
HKC-8 (kidney proximal tubular, human)
PU009-F (renal fibroblasts, human)
Kidney fibrosis modelNugraha et al., 2017
KCOT (keratocystic odontogenic tumor, human)
Cancer research, spheroid cultureNoguchi et al., 2017
3T3 (fibroblast, murine)Tisssue engineeringKoenig, G., et al., 2016
HCT-116 (colon carcinoma, human)Drug screeningRimann, M., Graf-Hausner, U., 2012
HeLa (adenocarcinoma, human)
HT1080 (epithelial cancer cell line from fibrosarcoma, human)
MDA-MB-231 (mamma carcinoma, human)
NES (neuroepithelial-like stem cell derived from H9 hESC, human)
Microarray Ueda, E., et al., 2012
C2C12 (myoblasts, murine)Cell-matrix interactions; mechanotransductionYin et al., 2021
Wang et al., 2019
PC9 (lung adenocarcinoma, human)Microsphere production, microsphere cultivation, scRNA-seq, fluorescent labeling of hydrogel via biotin-streptavidin attachment Bavli et al., 2021
Bavli et al., 2021
SiHa (human squamous cervical cancer cell line)
Tumor-stroma model on microfluidic platform; drug testing; tumor-on-chip Kromidas et al., 2023
Melanoma cell linesMigration assay Militaru et al., 2023
HaCaT (human keratinocytes)
Hs27 (human foreskin fibroblasts)
Full-thickness skin model Hafa et al., 2023
MDA-MB-231 (human breast cancer cell line)Tumor-on-chip model including T and CAR-T cells investigating extravasation, tumor infiltration and cytokine release; drug testing Maulana et al., 2023
HeLa Kyoto (adenocarcinoma, human)Investigation of interaction of cells with precisely immobilized proteins or peptides in 3D cell cultures. Krüger et al., 2022
Primary Cells
Fibroblasts (human)Tumor-stroma modelHensler et al., 2021
hMSCs (human mesenchymal stem cells)MechanotransductionWang et al., 2021
Benz, K., et al., 2010
ChondrocytesTissue engineering; cell-based therapeutic developmentLang et al., 2021
Rothdiener M. et al., 2018
Benz, K., et al., 2010
Pancreatic progenitor cells (human)Organoid cultureGeorgakopoulos et al., 2020
Cardiomyocytes (murine)MechanotransductionFriedrich et al., 2019
Friedrich, O. et al., 2017
PBMCs (human peripheral blood mononuclear cells)Lymph node model; perfusionKraus et al., 2019
APCs (antigen presenting cells)
DCs (dentritic cells)
ATSVF (Adipose tissue stromal vascular fraction)
BM-MSC (bone marrow mesenchymal stem cells)
ADSCs (adipose-derived stem cells)
UC-MSC (umbilical cord mesenchymal stem cells)
Lymph node model; perfusionSardi, M. et al., 2016
EPCs (endothelial progenitor cells, murine)Matrix-stiffness-dependent cell differentiation; hydrogel implantation in miceXue et al., 2019
Neurospheres (human)Neurotoxicity testingHellwig, C. et al., 2018
Nucleus pulposus (human)MechanotransductionHe et al., 2018
Intervertebral disk cells (human)Cell-based therapeutic developmentBenz, K., et al., 2010
Pancreatic islets (murine)Cell-based therapeutic development; implantationAyenehdeh et al., 2017
AT-MSCs (adipose tissue derived mesenchymal stem cells, murine)Cell-based therapeutic development; implantationAyenehdeh et al., 2017
HUVECs (Human Umbilical Vein Endothelial Cells)

Cell-based therapeutic development; implantation; angiogenesis Koenig, G., et al., 2016
Sun, J. et al., 2014
ZHBTc4 ESCs (mouse embryonic stem cells)Microsphere production, microsphere cultivation, scRNA-seq, fluorescent labeling of hydrogel via biotin-streptavidin attachment Bavli et al., 2021
Bavli et al., 2021
PMN (human polymorphonuclear leukocytes, neutrophils, from whole blood)
PBMCs (human peripheral blood mononucleated cells, from whole blood)
primary fibroblasts (from human cervix uteri)
combined with SiHa spheroids
Tumor-stroma model on microfluidic platform; drug testing Kromidas et al., 2023
Enteric neural stem cells, murineNeurosphere outgrowth; nervous system networksChen et al., 2023
HUVECs (Human Umbilical Vein Endothelial Cells)
Hs27 (human foreskin fibroblasts)
Angiogenesis model Hafa et al., 2023
Human breast cancer organoids (patient derived)
T cells, CAR-T cells (human)
Fibroblasts (human)
Tumor-on-chip model including T and CAR-T cells investigating extravasation, tumor infiltration and cytokine release; drug testing Maulana et al., 2023