|Yin et al. (2021) Cell migration regulated by spatially controlled stiffness inside composition-tunable three-dimensional dextran hydrogels. Adv. Mater. Interfaces 8, 2100494 (Link)|
The authors studied the regulation of cell migration by tuning the spatial stiffness of hydrogels. Migration velocity and cell morphology were analyzed to charaterize the dependence of cell migration on the heterogeneous stiffness in subcellular scale. Application: mechanotransduction. Cells Used: C2C12 murine myoblasts. Methods used: live/dead cell staining; cell tracking; rheological and atomic force microscopic measurements of hydrogels; scanning electron microscopy of hydrogels; fluorescent staining (phalloidin) and fluorescent antibody staining; fluorescence imaging of cells in gels.