|Wang et al. (2021) Spatial micro-variation of 3D hydrogel stiffness regulates the biomechanical properties of hMSCs. Biofabrication 13: 035051 (Article)|
The 3-D Life Hydrogel system was used to generate hydrogels with controlled variations in local stiffness at microscale dimensions to examine mechanotransductional effects on human mesenchymal stem cells. Application: cellular mechanotransduction. Cells Used: human mesenchymal stem cells (hMSCs). Methods used: atomic force microscopy; rheology; Live/dead staining; immunostaining; paraffin embedment and sectioning; hematoxylin-eosin staining; RT-qPCR.