Xue et al. (2019) Matrix stiffness regulates arteriovenous differentiation of endothelial progenitor cells during vasculogenesis in nude mice. Cell Proliferation 52:e12557 (Article)

The work shows how different stiffnesses of 3-D Life Hydrogels can direct the differentiation of endothelial progenitor cells (EPCs) into a venous or arterial phenotype. The gels injected into nude mice support the in vivo formation of functional blood vessels. Application: in vivo vasculogenesis. Cells used: murine endothelial progenitor cells. Methods used: hydrogel injection into mice; paraffin embedment of gels; antibody staining of tissue slices; dissolution of gels with dextranase and subsequent analysis of cells with qPCR and Western blotting.