3-D Life FG-Dextran

Catalog Number: M92-3

Unit Size: 3x 170 µl

Shipping Temperature: Ambient

Price: 160,00 €

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Description
Specifications

Details

Supporting Information

Description

3-D Life FG-Dextran is a component of the 3-D Life Hydrogel system. It contains maleimide-functionalized dextran (Mal-Dextran) that can be used together with PEG-Link (Cat. No.L50-1 or L50-3) or CD-Link (Cat. No. L60-1 or L60-3) to set up fast gelling, cell-compatible hydrogels.

Dextran-based 3-D Life hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

10 x CB (pH 7.2) is a buffer to control pH and osmotic conditions during gel formation in the presence of cells.

For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

M92-3

Application

3-D cell cultures; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.

Quantity

Allows formation of up to 6 ml 3-D Life Hydrogel depending on the stiffness of the gel, if used together with thiol-functionalized polymers (3-D Life PEG-Link or CD-Link).

Components

Material	Quantity	Concentration
Mal-Dextran, lyophilized	3 x 170 µl*	30 mmol/L* maleimide groups	MSDS
10x CB (pH 5.5)	600 µl	n.a.	MSDS
Water	4 x 1500 µl	n.a	MSDS
*Volume/concentration after reconstitution of lyophilisate.

3-D Life FG-Dextran

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Applications

User Guide

Data Sheet

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