fg90-1

3-D Life Dextran-PEG Hydrogel FG

Catalog Number: FG90-1

Unit Size: 1 Kit

Shipping Temperature: Ambient

Price: 89,00 €

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Supporting Information

Description The 3-D Life Dextran-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized dextran (Mal-Dextran) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-Dextran to provide a cell-adhesive matrix.

Dextran hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.
Catalog Number FG90-1
Application 3-D cell cultures requiring no cell spreading and migration; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.
Quantity Allows formation of up to 2 ml 3-D Life Hydrogel depending on the grade of stiffness of the gel.
Components
Material Quantity Concentration
Mal-Dextran, lyophilized170 µl*30 mmol/L* maleimide groupsMSDS
PEG-Link, lyophilized200 µl*20 mmol/L* thiol groupsMSDS
10x CB (pH 5.5)200 µln.aMSDS
Water2 x 1500 µln.aMSDS
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

Fast-gelling Hydrogels

Applications

Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels
Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels

User Guide

3-D Life Hydrogel User Guide

Data Sheet

Detailed Product Information

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