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3-D Life
PVA-CD Hydrogel SG
Catalog Number: G83-1
Unit Size: 1 Kit
Shipping Temperature: Ambient
Price:
194,00 €
How to Order
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Description
Specifications
Details
Supporting Information
Description
The
3-D Life
PVA-CD Hydrogel SG Kit provides reagents for setting up slow gelling, cell-compatible hydrogels. Its major components are polymeric
SG-PVA and the crosslinker CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with thiol-reactive groups on SG-PVA, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation.CD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala). The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9 [1]. It allows cells to spread and migrate within the hydrogel if they express the indicated MMPs. In most cases cell spreading and migration also requires the presence of adhesion peptides.Prior to the cross-linking step, peptides (e.g.
3-D Life
RGD Peptide, Cat.No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups of SG-PVA to provide a cell-adhesive matrix.For more information and instructions, please consult the General Protocol GP-2 "Preparation of
3-D Life
Slow Gelling Hydrogels" and the
3-D Life
Hydrogels User Guide on cellendes.com.
Catalog Number
G83-1
Application
3-D cell cultures
Quantity
Allows formation of up to 2 ml
3-D Life
Hydrogel depending on the stiffness of the gel.
Components
Material
Quantity
Concentration
SG-PVA
170 µl
30 mmol/L thioreactive groups
MSDS
CD-Link, lyophilized
200 µl*
20 mmol/L* thiol groups
MSDS
10x CB (pH 7.2)
200 µl
n.a.
MSDS
Water
2 x 1500 µl
n.a
MSDS
*Volume/concentration after reconstitution of lyophilisate.
General Protocols
Slow-gelling hydrogels
User Guide
3-D Life
Hydrogel User Guide
Data Sheet
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