3-D Life PVA-CD Hydrogel FG

Catalog Number: FG81-1

Unit Size: 1 Kit

Shipping Temperature: Dry Ice

Price: 194,00 €

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Description
Specifications

Details

Supporting Information

Description

The 3-D Life PVA-CD Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized polyvinyl aclcohol (Mal-PVA) and the crosslinker CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with maleimide groups on Mal-PVA, which results in the formation of the gel.

CD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala). The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9 [1]. It allows cells to spread and migrate within the hydrogel, if they express the indicated MMPs. In most cases cell spreading and migration also requires the presence of adhesion peptides.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat.No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-PVA to provide a cell-adhesive matrix.

For more information and instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

FG81-1

Application

3-D cell culture

Quantity

Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.

Components

Material	Quantity	Concentration
Mal-PVA	170 µl	30 mmol/L maleimide groups	MSDS
CD-Link, lyophilized	200 µl*	20 mmol/L* thiol groups	MSDS
10x CB (pH 5.5)	200 µl	n.a	MSDS
Water	2 x 1500 µl	n.a	MSDS
*Volume/concentration after reconstitution of lyophilisate.

3-D Life PVA-CD Hydrogel FG

General Protocols

User Guide

Data Sheet

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