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3-D Life
GFOGER-3 Peptide
Catalog Number: P12-1
Unit Size: 2.4 mg
Shipping Temperature: Ambient
Price:
120,00 €
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Description
Specifications
Details
Supporting Information
Description
The
3-D Life
GFOGER-3 Peptide spontaneously forms stable triple helices like those in fibrillar collagens including collagen type I, II, IV and XI [1-2]. Its amino acid sequence is derived from the triple helical building block, promoting cell adhesion through integrin receptors, including α1β1 and α2β1, α10β1, and α11β1 [3]. At each end the peptide is modified with a SH-group which binds to the SH-reactive group of
3-D Life
polymers. Once hydrogels are formed by crosslinking the polymers with
3-D Life
crosslinkers, the immobilized peptide promotes adhesion of cells carrying the appropriate integrin receptors. GFOGER-3 mediates cell adhesion within the gel or on top of the gel. For instructions of hydrogel preparations, please consult General Protocol GP-2 “Preparation of
3-D Life
Slow Gelling Hydrogels” and General Protocol GP-4 "Preparation of
3-D Life
Hyaluronic Acid Hydrogels". For general information consult the
3-D Life
Hydrogels User Guide.
Catalog Number
P12-1
Application
Modification of
3-D Life
Hydrogels with cell-adhesive properties. Culture of cells requiring a cell-adhesive matrix based on the GFOGER motif. Culture of cells in 3-D or on top of hydrogels.
Quantity
2.4 mg
Components
Material
Quantity
Concentration
GFOGER-3 Peptide, lyophilized
120 µl*
20 mg/ml*
MSDS
Water
600 µl
n/a
MSDS
* Volume/concentration after reconstitution of lyophilisate
Reference
[1] Khoshnoodi, J. et al. Microsc. Res. Tech. 71(5):357-370 (2008) [2] Siljander P. R.-M. et al. J Biol. Chem. 279(46):47763-47772 (2004) [3] Humphries, J. D. et al. J. Cell Sci. 119(19):3901-3903 (2006)
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