3-D Life Dextran-CD Hydrogel




Descriptions Picture The 3-D Life Dextran-CD Hydrogel Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized dextran (Mal-Dextran) and the crosslinker CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel.

CD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala). The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9 [1]. It allows cells to spread and migrate within the hydrogel if they express the indicated MMPs. In most cases cell spreading and migration also requires the presence of adhesion peptides.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-Dextran to provide a cell-adhesive matrix.

Dextran hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide on www.cellendes.com.
Catalog Number G91-1
Application 3-D cell cultures

Quantity Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.
Price 165 €
Components
Material Quantity Concentration
Mal-Dextran, lyophilized170 µl*30 mmol/L*MSDS
CD-Link, lyophilized200 µl*20 mmol/L*MSDS
10 x CB (pH 7.2)200 µln.a.MSDS
10 x CB (pH 5.5)200 µln.aMSDS
Thioglycerol200 µl20 mmol/LMSDS
Dextranase500 µln.a.MSDS
Water2 x 1500 µln.aMSDS
*Volume/concentration after reconstitution of lyophilisate.
Reference[1] Knight, C. G. et al. FEBS 296:263-66 (1992)