|| The 3-D Life Dextran-PEG Hydrogel Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized dextran (Mal-Dextran) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel. Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-Dextran to provide a cell-adhesive matrix. Dextran hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide on www.cellendes.com.
||3-D cell cultures requiring no cell spreading and migration; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.
Allows formation of up to 2 ml 3-D Life Hydrogel depending on the grade of stiffness of the gel.
|Mal-Dextran, lyophilized||170 µl*||30 mmol/L*||MSDS|
|PEG-Link, lyophilized||200 µl*||20 mmol/L*||MSDS|
|10 x CB (pH 7.2)||200 µl||n.a.||MSDS|
|10 x CB (pH 5.5)||200 µl||n.a||MSDS|
|Thioglycerol||200 µl||20 mmol/L||MSDS|
|Water||2 x 1500 µl||n.a||MSDS|
|*Volume/concentration after reconstitution of lyophilisate.