3-D Life Dextran-PEG Hydrogel




Descriptions Picture The 3-D Life Dextran-PEG Hydrogel Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized dextran (Mal-Dextran) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-Dextran to provide a cell-adhesive matrix.

Dextran hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide on www.cellendes.com.
Catalog Number G90-1
Application 3-D cell cultures requiring no cell spreading and migration; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.
Quantity Allows formation of up to 2 ml 3-D Life Hydrogel depending on the grade of stiffness of the gel.
Price 65 €
Components
Material Quantity Concentration
Mal-Dextran, lyophilized170 µl*30 mmol/L*MSDS
PEG-Link, lyophilized200 µl*20 mmol/L*MSDS
10 x CB (pH 7.2)200 µln.a.MSDS
10 x CB (pH 5.5)200 µln.aMSDS
Thioglycerol200 µl20 mmol/LMSDS
Dextranase500 µln.a.MSDS
Water2 x 1500 µln.aMSDS
*Volume/concentration after reconstitution of lyophilisate.